Apoptosis induced by aminoglycosides in LLC-PK1 Cells: comparative study of neomycin, gentamicin, amikacin, and isepamicin using electroporation.
نویسندگان
چکیده
Levels of apoptosis induction (4',6'-diamidino-2-phenylindole staining, activation of caspase 3) for aminoglycosides were compared by using renal LLC-PK1 cells. Amikacin caused less apoptosis than gentamicin in incubated cells. In electroporated cells, neomycin B and gentamicin caused apoptosis in the 0.03 to 0.1 mM range, isepamicin required larger concentrations (0.2 mM), and amikacin was without effect.
منابع مشابه
Apoptosis induced by aminoglycosides in LLC-PK1 cells: comparison between neomycin, gentamicin, amikacin, and isepamicin using electroporation
* Corresponding author: Mailing address: UCL 7370 Ave. E. Mounier 73 B-1200 Brussels, Belgium. Phone: +32-2-762-2136. Fax: +32-2-764-7373. E-mail: [email protected] AC CE PT ED Copyright © 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved. Antimicrob. Agents Chemother. doi:10.1128/AAC.01680-07 AAC Accepts, published online ahead of print on...
متن کاملApoptosis in renal proximal tubules of rats treated with low doses of aminoglycosides.
Kidney cortex apoptosis was studied with female Wistar rats treated for 10 days with gentamicin and netilmicin at daily doses of 10 or 20 mg/kg of body weight and amikacin or isepamicin at daily doses of 40 mg/kg. Apoptosis was detected and quantitated using cytological (methyl green-pyronine) and immunohistochemical (terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling) ...
متن کاملGentamicin causes apoptosis at low concentrations in renal LLC-PK1 cells subjected to electroporation.
Gentamicin accumulates in the lysosomes of kidney proximal tubular cells and causes apoptosis at clinically relevant doses. Gentamicin-induced apoptosis can be reproduced with cultured renal cells, but only at high extracellular concentrations (1 to 3 mM; 0.4 to 1.2 g/liter) because of its low level of uptake. We recently showed that gentamicin-induced apoptosis in LLC-PK1 cells involves a rapi...
متن کاملIn vitro activity of isepamicin and other aminoglycosides against clinical isolates of Gram-negative bacteria causing nosocomial bloodstream infections.
BACKGROUND AND PURPOSE Isepamicin is a newly introduced aminoglycoside in Taiwan. Since in vitro data for isepamicin against nosocomial Gram-negative bloodstream infection from Taiwan are limited, we compared the activity of isepamicin, amikacin, gentamicin and tobramycin against nosocomial Gram-negative blood isolates. METHODS A total of 247 non-duplicate nosocomial blood isolates of Gram-ne...
متن کاملGentamicin Induced Apoptosis of Renal Tubular Epithelial (LLC-PK1) Cells
Nephrotoxicity is a major limiting factor in the use of aminoglycoside antibiotics, the mechanisms for which are still speculative. To clarify the mechanisms of renal tubular cell death induced by aminoglycosides, we examined the renal proximal tubule-like cell line, LLC-PK1, after inducing apoptosis through a chronic treatment with gentamicin (GM). Changes in the expression of the Fas were als...
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ورودعنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 52 6 شماره
صفحات -
تاریخ انتشار 2008